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Preparation, characterization, and evaluation of genipin crosslinked chitosan/gelatin three‐dimensional scaffolds for liver tissue engineering applications
Author(s) -
Zhang Yi,
Wang QiangSong,
Yan Kuo,
Qi Yun,
Wang GuiFang,
Cui YuanLu
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35717
Subject(s) - genipin , biocompatibility , gelatin , chitosan , materials science , tissue engineering , glutaraldehyde , biomedical engineering , biomaterial , scanning electron microscope , chemical engineering , nanotechnology , chemistry , composite material , organic chemistry , medicine , engineering , metallurgy
In liver tissue engineering, scaffolds with porous structure desgined to supply nutrient and oxygen exchange for three‐dimensional (3‐D) cells culture, and maintain liver functions. Meanwhile, genipin, as a natural crosslinker, is widely used to crosslink biomaterials in tissue engineering, with lower cytotoxicity and better biocompatibility. In present study, chitosan/gelatin 3‐D scaffolds crosslinked by genipin, glutaraldehyde or 1‐(3‐dimethylaminopropyl)−3‐ethyl‐carbodimide hydrochloride (EDC) were prepared and characterized by Fourier‐transform infrared (FT‐IR) and scanning electron microscopy (SEM). The biocompatibility of chitosan/gelatin scaffolds corsslinked with different crosslinkers was investigated by cell viability, morphology and liver specific functions. The result showed that the 1% and 2% genipin crosslinked chitosan/gelatin scaffolds possess ideal porosity. The genipin crosslinked 3‐D scaffolds possessed the best biocompatibility than that of the others, and maintained liver specific functions when HepG2 cells seeded on scaffolds. The cellular morphology of HepG2 cells seeded on scaffolds showed that cells could penetrate into the scaffolds and proliferate significantly. Therefore, genipin crosslinked chitosan/gelatin scaffolds could be a promising biomaterial used in liver tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1863–1870, 2016.