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Extracellular matrix components supporting human islet function in alginate‐based immunoprotective microcapsules for treatment of diabetes
Author(s) -
Llacua Alberto,
de Haan Bart J.,
Smink Sandra A.,
de Vos Paul
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35706
Subject(s) - islet , extracellular matrix , laminin , pancreatic islets , transplantation , pancreas , microbiology and biotechnology , extracellular , tissue engineering , materials science , endocrinology , insulin , biology , medicine , biomedical engineering
Abstract In the pancreas, extracellular matrix (ECM) components play an import role in providing mechanical and physiological support, and also contribute to the function of islets. These ECM‐connections are damaged during islet‐isolation from the pancreas and are not fully recovered after encapsulation and transplantation. To promote the functional survival of human pancreatic islets, we tested different ECMs molecules in alginate‐encapsulated human islets. These were laminin derived recognition sequences, IKVAV, RGD, LRE, PDSGR, collagen I sequence DGEA (0.01 − 1.0 m M ), and collagen IV (50 − 200 µg/mL). Interaction with RGD and PDSGR promoted islet viability and glucose induced insulin secretion (GIIS) when it was applied at concentrations ranging from 0.01 − 1.0 m M ( p < 0.05). Also the laminin sequence LRE contributed to enhanced GIIS but only at higher concentrations of 1 mM ( p < 0.05). Collagen IV also had beneficial effects but only at 50 µg/ml and no further improvement was observed at higher concentrations. IKVAV and DGEA had no effects on human islets. Synergistic effects were observed by adding Collagen(IV)‐RGD, Collagen(IV)‐LRE, and Collagen(IV)‐PDSGR to encapsulated human islets. Our results demonstrate the potential of specific ECM components in support of functional survival of human encapsulated and free islet grafts. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1788–1796, 2016.