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In vitro osteogenic induction of human marrow‐derived mesenchymal stem cells by PCL fibrous scaffolds containing dexamethazone‐loaded chitosan microspheres
Author(s) -
Omidvar Noushin,
Ganji Fariba,
Eslaminejad Mohamadreza Baghaban
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35695
Subject(s) - materials science , chitosan , mesenchymal stem cell , in vitro , biomedical engineering , microsphere , stem cell , bone marrow , chemical engineering , microbiology and biotechnology , medicine , immunology , chemistry , biology , biochemistry , engineering
This research reports the encapsulation of dexamethasone (Dex) within the chitosan microspheres (CSMs) embedded in a fibrous structure of poly(ɛ‐caprolactone) (PCL) to provide a platform for osteogenic differentiation of human mesenchymal stem cells (hMSCs). Dex loaded CSMs were prepared by spray drying a mixture of chitosan and Dex. Then, they were electrospun with PCL solution to create a bilayer fibrous scaffold (PCL/CSMs‐Dex). The CSMs act as good depots for sustained release of Dex over a period of 14 days, without noticeable burst release. This is mainly attributed to the core‐shell structure of the final PCL/CSMs‐Dex‐matrix, which could prolong the release and eliminate the initial burst. The water contact angle of PCL scaffolds decreased from 141.4 ± 3.8 to 118.4 ± 7.6 in the presence of CSMs. Improved proliferation of hMSCs cultured on PCL/CSMs‐Dex scaffolds was also evidenced. Furthermore, osteogenic assays showed an increase in alkaline phosphatase activity and mineral deposits. The expression of bone‐specific genes also confirmed the osteogenic differentiation of cells cultured on these Dex‐loaded core‐shell structures. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1657–1667, 2016.