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Near‐infrared fluorescent peptide probes for imaging of tumor in vivo and their biotoxicity evaluation
Author(s) -
Liu Liwei,
Lin Guimiao,
Yin Feng,
Law WingCheung,
Yong KenTye
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35628
Subject(s) - in vivo , peptide , fluorescence , materials science , angiogenesis , preclinical imaging , molecular probe , fluorescence lifetime imaging microscopy , molecular imaging , biomedical engineering , cancer research , pathology , chemistry , medicine , biology , biochemistry , optics , dna , physics , microbiology and biotechnology
Optical imaging techniques are becoming increasingly urgent for the early detection and monitoring the progression of tumor development. However, tumor vasculature imaging has so far been largely unexplored because of the lack of suitable optical probes. In this study, we demonstrated the preparation of near‐infrared (NIR) florescent RGD peptide probes for noninvasive imaging of tumor vasculature during tumor angiogenesis. The peptide optical probes combined the advantages of NIR emission and RGD peptide, which possesses minimal biological absorption and specially targets the integrin, which highly expressed on activated tumor endothelial cells. In vivo optical imaging of nude mice bearing pancreatic tumor showed that systemically delivered NIR probes enabled us to visualize the tumors at 24 hours post‐injection. In addition, we have performed in vivo toxicity study on the prepared fluorescent RGD peptide probes formulation. The blood test results and histological analysis demonstrated that no obvious toxicity was found for the mice treated with RGD peptide probes for two weeks. These studies suggest that the NIR fluorescent peptide probes can be further designed and employed for ultrasensitive fluorescence imaging of angiogenic tumor vasculature, as well as imaging of other pathophysiological processes accompanied by activation of endothelial cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 910–916, 2016.