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Layer‐by‐layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery
Author(s) -
Bishop Corey J.,
Liu Allen L.,
Lee David S.,
Murdock Richard J.,
Green Jordan J.
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35610
Subject(s) - gene delivery , materials science , nanotechnology , nanoparticle , layer (electronics) , gene , plasmid , gene expression , genetic enhancement , computational biology , biology , genetics
Nonviral gene delivery methods represent a potential safe and effective approach for treating myriad diseases. For many gene therapy applications, delivering multiple exogenous genes and controlling the time profile that these genes are expressed would be advantageous. Polymeric nonviral gene carriers are versatile and can be readily tailored for particular therapeutic applications, have the ability to carry multiple large genes within each particle, and can be more easily manufactured than viruses used for gene delivery. A layer‐by‐layer (LbL) theranostic‐enabling nanoparticle was developed to incorporate two plasmid types which have differing expression time profiles. Temporally controlling the expression of exogenous DNA enables superior control over the microenvironment and could lead to better control over differentiation pathways and cell fate. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 707–713, 2016.