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Tailoring sub‐micron PLGA particle release profiles via centrifugal fractioning
Author(s) -
Dutta Dipankar,
Salifu Mariama,
Sirianni Rachael W.,
Stabenfeldt Sarah E.
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35608
Subject(s) - plga , materials science , particle size , population , particle (ecology) , nanoparticle , particle size distribution , drug delivery , biomedical engineering , composite material , nanotechnology , chemical engineering , medicine , oceanography , demography , sociology , geology , engineering
Poly(D,L‐lactic‐ co ‐glycolic) acid (PLGA)‐based sub‐micron particles are uniquely posed to overcome limitations of conventional drug delivery systems. However, tailoring cargo/payload release profiles from PLGA micro/nanoparticles typically requires optimization of the multi‐parameter formulation, where small changes may cause drastic shifts in the resulting release profiles. In this study, we aimed to establish whether refining the average diameter of sub‐micron particle populations after formulation alters protein release profiles. PLGA particles were first produced via double emulsion‐solvent evaporation method to encapsulate bovine serum albumin. Particles were then subjected to centrifugal fractioning protocols varying in both spin time and force to determine encapsulation efficiency and release profile of differently sized populations that originated from a single batch. We found the average particle diameter was related to marked alterations in encapsulation efficiencies (range: 36.4–49.4%), burst release (range: 15.8–49.1%), and time for total cargo release (range: 38–78 days). Our data corroborate previous reports relating PLGA particle size with such release characteristics, however, this is the first study, to our knowledge, to directly compare particle population size while holding all formulation parameters constant. In summary, centrifugal fractioning to selectively control the population distribution of sub‐micron PLGA particles represents a feasible tool to tailor release characteristics. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A 104A: 688–696, 2016.