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Modifying alginate with early embryonic extracellular matrix, laminin, and hyaluronic acid for adipose tissue engineering
Author(s) -
Chen YoShen,
Chen YenYu,
Hsueh YuSheng,
Tai HaoChih,
Lin FengHuei
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35606
Subject(s) - hyaluronic acid , extracellular matrix , biocompatibility , materials science , tissue engineering , adipogenesis , adipose tissue , cell adhesion , adhesion , laminin , cell growth , embryonic stem cell , cell , microbiology and biotechnology , biophysics , biomedical engineering , biochemistry , chemistry , biology , anatomy , composite material , medicine , gene , metallurgy
Extracellular matrix provides both mechanistic and chemical cues that can influence cellular behaviors such as adhesion, migration, proliferation, and differentiation. In this study, a new material, HA‐L‐Alg, was synthesized by linking developmentally essential ECM constituents hyaluronic acid (HA) and laminin(L) to alginate (Alg). The fabrication of HA‐L‐Alg was confirmed by FTIR spectroscopy, and it was used to form 3D cell‐carrying beads. HA‐L‐Alg beads had a steady rate of degradation and retained 63.25% of mass after 9 weeks. HA‐L‐Alg beads showed biocompatibility comparable to beads formed by Alg‐only with no obvious cytotoxic effect on the embedded 3T3‐L1 preadipocytes. HA‐L‐Alg encapsulated 3T3‐L1 cells were found to have a higher proliferation rate over those in Alg‐only beads. These cells also showed better differentiation capacity after 2 weeks of adipogenic induction within HA‐L‐Alg beads. These results support that HA‐L‐Alg facilitated cell survival and proliferation, as well as stimulated and maintained cell differentiation. Our results suggest that HA‐L‐Alg has a great clinical potential to be used as stem cell carrier for adipose tissue engineering. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 669–677, 2016.