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In vitro mineralization of MC3T3‐E1 osteoblast‐like cells on collagen/nano‐hydroxyapatite scaffolds coated carbon/carbon composites
Author(s) -
Cao Sheng,
Li Hejun,
Li Kezhi,
Lu Jinhua,
Zhang Leilei
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35593
Subject(s) - materials science , osteoblast , mineralization (soil science) , in vitro , composite material , nano , carbon fibers , biomedical engineering , biophysics , composite number , biochemistry , chemistry , organic chemistry , medicine , nitrogen , biology
Collagen/nano‐hydroxyapatite (collagen/nHA) scaffolds were successfully prepared on carbon/carbon composites as bioactive films using the layer‐by‐layer coating method. Surface characterizations of collagen/nHA scaffolds were detected by scanning electron microscope (SEM), X‐ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Compressive strengths of the scaffolds were evaluated by a universal test machine. In vitro biological performances were determined using scaffolds seeded with MC3T3‐E1 osteoblasts‐like cells and cultured in mineralization medium for up to 21 days. In addition, cellular morphologies and several related gene expressions of MC3T3‐E1 cells in the scaffolds were also evaluated. Chemical and morphological analysis showed that the scaffolds had uniform pore sizes and unified phase composition. Mechanical testing indicated that the collagen/nHA scaffolds had the highest compressive strength in 50% of strain condition when the proportion of collagen and nano‐hydroxyapatite was 1:3. Cellular morphology observations and cytology tests indicated that MC3T3‐E1 cells were adhered on these scaffolds and proliferated. SEM photographs and gene expressions showed that mineralized MC3T3‐E1 cells and newly formed extra cellular matrix (ECM) filled up the pores of the scaffolds after the 3‐week mineralization inducement. Nano‐sized apatite particles were secreted from MC3T3‐E1 cells and combined with the reconstructed ECM. Collectively, collagen/nHA scaffolds provided C/C composites with a biomimetic surface for cell adhesion, proliferation and mineralized extra cellular matrices formation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 533–543, 2016.