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The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering
Author(s) -
Chuenjitkuntaworn Boontharika,
Osatha Thanaphum,
Nowwarote Nunthawan,
Supaphol Pitt,
Pavasant Prasit
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35558
Subject(s) - mesenchymal stem cell , dental pulp stem cells , scaffold , stem cell , materials science , biomedical engineering , tissue engineering , polycaprolactone , alkaline phosphatase , mtt assay , microbiology and biotechnology , biocompatibility , viability assay , chemistry , cell growth , cell , biology , medicine , biochemistry , metallurgy , composite material , enzyme , polymer
Major drawbacks of using an autograft are the possibilities of insufficient bony source and patient's morbidity after operation. Bone tissue engineering technology, therefore, has been applied for repairing bony defects. Previous study showed that a novel fabricated 3D‐Polycaprolactone/Hydroxyapatite (PCL/HAp) scaffold possessed a good biocompatibility for bone cells. This study aimed to determine the ability of PCL/HAp for supporting cell growth, gene expression, and osteogenic differentiation in three types of mesenchymal stem cells, including bone marrow‐derived mesenchymal stem cells (BMSCs), dental pulp stem cells (DPSCs), and adiposed‐derived mesenchymal stem cells (ADSCs). These were assessed by cell viability assay (MTT), reverse‐transcription polymerase chain reaction (RT‐PCR) analysis, alkaline phosphatase activity, and osteogenic differentiation by alizarin red‐S staining. The results showed that PCL/HAp scaffold could support growth of all three types of mesenchymal stem cells. In addition, DPSCs with PCL/HAp showed the highest level of calcium deposition compared to other groups. In conclusion, DPSCs exhibited a better compatibility with these scaffolds compared to BMSCs and ADSCs. However, the PCL/HAp could be a good candidate scaffold for all tested mesenchymal stem cells in bone tissue engineering. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: A: 264–271, 2016.