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Cellular activity of W harton's J elly‐derived mesenchymal stem cells on electrospun fibrous and solvent‐cast film scaffolds
Author(s) -
Bagher Zohreh,
EbrahimiBarough Somayeh,
Azami Mahmoud,
Safa Majid,
Joghataei Mohammad Taghi
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35555
Subject(s) - mesenchymal stem cell , polycaprolactone , adhesion , materials science , scaffold , biomedical engineering , tissue engineering , viability assay , microbiology and biotechnology , chemistry , cell , composite material , biochemistry , biology , polymer , medicine
It was shown that topography and surface chemistry of materials influence cell behaviors. In this study, the effects of chemistry and topography of scaffold surface on adhesion, proliferation and differentiation of Wharton's Jelly mesenchymal (WJMSCs) stem cells into motor neurons were investigated. WJMSCs were cultivated in an neurogenic inductive medium on the surface of modified and unmodified polycaprolactone (PCL) electrospun fibrous and solvent‐cast film scaffolds. All the scaffolds were characterized according to their ability to support cell attachment and viability by SEM and MTT assay. The expression of motor neuron‐specific markers was assayed by real‐time PCR after 15days post induction. Results showed that attachment, proliferation and differentiation of WJMSCs into motor neuron‐like cells on the nanotopographic surface was higher than that of the cells on the solvent‐cast scaffolds. In addition, regardless of their topography, WJMSCs cultured on collagen‐coated PCL nanofibrous showed results similar to collagen‐coated PCL films. Results suggested that surface chemistry has more impact on WJMSCs behaviour rather than topography. In conclusion, collagen‐coated electrospun PCL have potential for being used in neural tissue engineering because of its bioactive and three‐dimensional structure which enhance viability and differentiation of WJMSCs. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 218–226, 2016.