Premium
T i O 2 nanoparticles‐induced apoptosis of primary cultured Sertoli cells of mice
Author(s) -
Hong Fashui,
Zhao Xiaoyang,
Chen Ming,
Zhou Yingjun,
Ze Yuguan,
Wang Ling,
Wang Yajing,
Ge Yushuang,
Zhang Qi,
Ye Lingqun
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35548
Subject(s) - sertoli cell , cytochrome c , apoptosis , microbiology and biotechnology , reactive oxygen species , viability assay , downregulation and upregulation , oxidative stress , superoxide dismutase , biology , mitochondrion , biochemistry , endocrinology , spermatogenesis , gene
Abstract Titanium dioxide nanoparticles (TiO 2 NPs), as largest production and use of nanomaterials, have been demonstrated to have a potential toxicity on reproductive system. However, the mechanism underlying male reproductive toxicity of TiO 2 NPs remains limited. Thus, our study was designed to examine the cellular viability, apoptosis, oxidative stress, antioxidant capacity, and expression of apoptotic cytokines in primary cultured Sertoli cells isolated from mice under TiO 2 NPs exposure. Results showed that TiO 2 NPs exposure from 5 to 30 μg/mL resulted in reduction of cell viability, lactate dehydrogenase release, and induction of apoptosis or death on Sertoli cells. TiO 2 NPs could migrate to Sertoli cells, which induced mitochondria‐mediated or endoplasmic‐reticulum‐mediated apoptotic changes including elevation in reactive oxygen species (ROS) generation and reductions in superoxide dismutase, catalase, and glutathione peroxidase activities, decreases in mitochondrial membrane potential (ΔΨm), and releases of cytochrome c into the cytosol. In addition, upregulation of cytochrome c, Bax, caspase‐3, glucose‐regulated protein 78, and C/EBP homologous protein and caspase‐12 protein expression, and downregulation of bcl‐2 protein expression in primary cultured Sertoli cells induced by TiO 2 NPs treatment. All of the results suggested that ROS generation may play a critical role in the initiation of TiO 2 NPs‐induced apoptosis by mediation of the disruption of ΔΨm, the cytochrome c release, and further the activation of caspase cascade and unfolded protein response signaling pathway. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 124–135, 2016.