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Controlled release of an anthrax toxin‐neutralizing antibody from hydrolytically degradable polyethylene glycol hydrogels
Author(s) -
Liang Yingkai,
Coffin Megan V.,
Manceva Slobodanka D.,
Chichester Jessica A.,
Jones R. Mark,
Kiick Kristi L.
Publication year - 2016
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35545
Subject(s) - self healing hydrogels , peg ratio , anthrax toxin , ethylene glycol , polyethylene glycol , materials science , in vitro , controlled release , monoclonal antibody , antibody , polymer , biophysics , polymer chemistry , biochemistry , chemistry , nanotechnology , organic chemistry , biology , immunology , recombinant dna , finance , economics , fusion protein , gene , composite material
In this study, hydrophilic and hydrolytically degradable poly (ethylene glycol) (PEG) hydrogels were formed via Michael‐type addition and employed for sustained delivery of a monoclonal antibody against the protective antigen of anthrax. Taking advantage of the PEG‐induced precipitation of the antibody, burst release from the matrix was avoided. These hydrogels were able to release active antibodies in a controlled manner from 14 days to as long as 56 days in vitro by varying the polymer architectures and molecular weights of the precursors. Analysis of the secondary and tertiary structure and the in vitro activity of the released antibody showed that the encapsulation and release did not affect the protein conformation or functionality. The results suggest the promise for developing PEG‐based carriers for sustained release of therapeutic antibodies against toxins in various applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 113–123, 2016.