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Cell response to hydroxyapatite surface topography modulated by sintering temperature
Author(s) -
Mealy Jacob,
O'Kelly Kevin
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35487
Subject(s) - materials science , resazurin , sintering , viability assay , mesenchymal stem cell , cell , in vivo , biophysics , diffusion , biomedical engineering , wavelength , composite material , microbiology and biotechnology , biochemistry , optoelectronics , chemistry , biology , medicine , physics , thermodynamics
Increased mesenchymal stem cell (MSC) activity on hydroxyapatite (HA) bone tissue engineering scaffolds will improve their viability in diffusion‐based in vivo environments and is therefore highly desirable. This work focused on modulating the sintered HA surface topography with a view to increasing cell activity; this was achieved by varying the sintering temperature of the HA substrates. Cells were cultured on the substrates for periods of up to 19 days and displayed a huge variation in viability. MSC metabolic activity was measured using a resazurin sodium salt assay and revealed that surfaces sintered from 1250 to 1350°C significantly outperformed their lower temperature counterparts from day one ( p ≤ 0.05). Surfaces sintered at 1300°C induced 57% more cell activity than the control at day 16. No significant activity was observed on surfaces sintered below 1200°C. It is suggested that this is due to the granular morphology produced at these temperatures providing insufficient contact area for cell attachment. In addition, we propose the average surface wavelength as a more quantitative surface descriptor than those readily found in the literature. The wavelengths of the substrates presented here were highly correlated with cell activity ( R 2 = 0.9019); with a wavelength of 2.675 µm on the 1300°C surface inducing the highest cell response. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3533–3538, 2015.