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Biological activation of zirconia surfaces by chemical modification with IGF‐1
Author(s) -
Ito Daisuke,
Kado Takashi,
NaganoTakebe Futami,
Hidaka Tatsuhiro,
Endo Kazuhiko,
Furuichi Yasushi
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35476
Subject(s) - adhesion , materials science , streptococcus gordonii , x ray photoelectron spectroscopy , cubic zirconia , fourier transform infrared spectroscopy , biofilm , surface modification , cell adhesion , nuclear chemistry , chemical engineering , composite material , bacteria , chemistry , ceramic , biology , engineering , genetics
The purpose of this study was to improve the adhesion and extension of human gingival epithelial cells (HGECs) to the yttria‐stabilized zirconia polycrystal (Y‐TZP) surfaces by immobilization of insulin‐like growth factor 1 (IGF‐1). Surface analyses by Fourier transform infrared spectroscopy (FTIR) and X‐ray photoelectron spectroscopy (XPS) showed that IGF‐1 was successfully immobilized on the Y‐TZP surfaces. There was no significant difference between the number of cells attached to the IGF‐1‐immobilized Y‐TZP surfaces and on the as‐polished Y‐TZP surfaces either at 3 or 72 h. However, IGF‐1‐immobilized Y‐TZP surfaces yielded a significantly higher expression of integrin β4 mRNA and laminin‐5 mRNA, and enhanced adhesion strength of HGECs after 72 h of incubation. There was no difference between the amount of adhered Streptococcus gordonii ( S. gordonii ) found on the IGF‐1‐immobilized Y‐TZP surfaces and on the as‐polished Y‐TZP surfaces. These results suggested that the IGF‐1‐immobilized Y‐TZP surfaces developed using the method reported herein enhanced the adhesion and extension of HGECs to the Y‐TZP surfaces without enhancing S. gordonii adhesion. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3659–3665, 2015.