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Osteoblast biocompatibility of premineralized, hexamethylene‐1,6‐diaminocarboxysulfonate crosslinked chitosan fibers
Author(s) -
Kiechel Marjorie A.,
Beringer Laura T.,
Donius Amalie E.,
Komiya Yuko,
Habas Raymond,
Wegst Ulrike G. K.,
Schauer Caroline L.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35451
Subject(s) - ultimate tensile strength , biocompatibility , materials science , electrospinning , composite material , composite number , chitosan , biopolymer , fiber , tensile testing , genipin , tissue engineering , chemical engineering , biomedical engineering , polymer , medicine , engineering , metallurgy
Biopolymer–ceramic composites are thought to be particularly promising materials for bone tissue engineering as they more closely mimic natural bone. Here, we demonstrate the fabrication by electrospinning of fibrous chitosan‐hydroxyapatite composite scaffolds with low (1 wt %) and high (10 wt %) mineral contents. Scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS) and unidirectional tensile testing were performed to determine fiber surface morphology, elemental composition, and tensile Young's modulus ( E ) and ultimate tensile strength ( σ UTS ), respectively. EDS scans of the scaffolds indicated that the fibers, crosslinked with either hexamethylene‐1,6‐diaminocarboxysulfonate (HDACS) or genipin, have a crystalline hydroxyapatite mineral content at 10 wt % additive. Moreover, FESEM micrographs showed that all electrospun fibers have diameters (122–249 nm), which fall within the range of those of fibrous collagen found in the extracellular matrix of bone. Young's modulus and ultimate tensile strength of the various crosslinked composite compositions were in the range of 116–329 MPa and 2–15 MPa, respectively. Osteocytes seeded onto the mineralized fibers were able to demonstrate good biocompatibility enhancing the potential use for this material in future bone tissue engineering applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3201–3211, 2015.