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The significance of grafting collagen on polycaprolactone composite scaffolds: Processing–structure–functional property relationship
Author(s) -
Kiran S.,
Nune K. C.,
Misra R. D. K.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35431
Subject(s) - polycaprolactone , materials science , scaffold , glutaraldehyde , tissue engineering , polyester , cell adhesion , biomedical engineering , adhesion , chemical engineering , polymer , composite material , organic chemistry , chemistry , medicine , engineering
The study concerns processing–structure–functional property relationship in organic–inorganic hybrid scaffolds based on grafted collagen for bone tissue engineering. Biodegradable polyester, polycaprolactone (PCL) and nanohydroxyapatite were used to fabricate three‐dimensional porous scaffolds by adopting a combination of solvent casting, particulate leaching, and polymer leaching approaches. The PCL scaffold was subsequently surface modified by chemical bonding of 1,6‐hexanediamine to the ester groups of PCL to introduce free NH 2 groups. The introduction of NH 2 groups as active sites enabled immobilization of biocompatible macromolecule, collagen, on the aminolyzed PCL via a cross‐linking agent, glutaraldehyde. The osteoblasts' functions, notably cell adhesion, proliferation, and mineralization, were favorably modulated because of the chemical interaction between Arg‐Gly‐Asp domains in collagen molecule and integrin receptor in the cell membrane. The study underscores the significance of grafting collagen on PCL‐nHA scaffold in modulating cellular activity and biological functions expanding its current use in soft tissue engineering to hard tissue regeneration. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2919–2931, 2015.