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I n vitro and in vivo study of hazardous effects of Ag nanoparticles and Arginine‐treated multi walled carbon nanotubes on blood cells: A pplication in hemodialysis membranes
Author(s) -
ZareZardini Hadi,
Amiri Ahmad,
Shanbedi Mehdi,
TaheriKafrani Asghar,
Kazi S. N.,
Chew B.T.,
Razmjou Amir
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35425
Subject(s) - materials science , hematocrit , nanoparticle , nuclear chemistry , carbon nanotube , in vivo , thermogravimetric analysis , fourier transform infrared spectroscopy , membrane , nanotechnology , chemical engineering , chemistry , medicine , organic chemistry , biochemistry , biology , microbiology and biotechnology , endocrinology , engineering
One of the novel applications of the nanostructures is the modification and development of membranes for hemocompatibility of hemodialysis. The toxicity and hemocompatibility of Ag nanoparticles and arginine‐treated multiwalled carbon nanotubes (MWNT‐Arg) and possibility of their application in membrane technology are investigated here. MWNT‐Arg is prepared by amidation reactions, followed by characterization by FTIR spectroscopy, Raman spectroscopy, and thermogravimetric analysis. The results showed a good hemocompatibility and the hemolytic rates in the presence of both MWNT‐Arg and Ag nanoparticles. The hemolytic rate of Ag nanoparticles was lower than that of MWNT‐Arg. In vivo study revealed that Ag nanoparticle and MWNT‐Arg decreased Hematocrit and mean number of red blood cells (RBC) statistically at concentration of 100 µg mL −1 . The mean decrease of RBC and Hematocrit for Ag nanoparticles (18% for Hematocrit and 5.8 × 1,000,000/µL) was more than MWNT‐Arg (20% for Hematocrit and 6 × 1/µL). In addition, MWNT‐Arg and Ag nanoparticles had a direct influence on the White Blood Cell (WBC) drop. Regarding both nanostructures, although the number of WBC increased in initial concentration, it decreased significantly at the concentration of 100 µg mL −1 . It is worth mentioning that the toxicity of Ag nanoparticle on WBC was higher than that of MWNT‐Arg. Because of potent antimicrobial activity and relative hemocompatibility, MWNT‐Arg could be considered as a new candidate for biomedical applications in the future especially for hemodialysis membranes. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2959–2965, 2015.