Premium
Effect of G‐CSF on the osteoinductive property of a BCP/blood clot composite
Author(s) -
Paul Adrien J.,
Momier David,
Boukhechba Florian,
Michiels JeanFrançois,
Lagadec Patricia,
Rochet Nathalie
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35424
Subject(s) - haematopoiesis , mesenchymal stem cell , progenitor cell , granulocyte colony stimulating factor , granulocyte , materials science , biomaterial , stem cell , blood cell , microbiology and biotechnology , immunology , pharmacology , biomedical engineering , medicine , biology , chemotherapy
We previously reported that blood clot combined with biphasic calcium phosphate microparticles constitute a biomaterial (BRB) that can repair a bone critical defect in rat and induces subcutaneous bone formation in mice. The granulocyte colony‐stimulating factor (G‐CSF) is the agent most commonly used in human to enrich blood with hematopoietic stem and progenitor cells (HSPCs) as well as granulocytes (GCs). Moreover, recent data also suggest that it can mobilize mesenchymal stem cells (MSCs). Here, we asked whether the osteoinductive properties of the BRB could be further enhanced by G‐CSF, either by replacing normal blood by G‐CSF‐mobilized blood (BRBe) or by treating the recipient animals with G‐CSF. The experiments performed in C57BL/6 mice showed that G‐CSF induces a marked increase of circulating HPCs and GCs, but not of MSCs. BRBe prepared with G‐CSF‐enriched blood induced a slight but significant decrease of subcutaneous bone formation compared to BRB prepared with normal blood. Additional injection of G‐CSF to the recipient mice had no significant effect on the bone formation induced by BRB or BRBe. Altogether these results indicate that, in this model of ectopic implantation, cell mobilization induced by G‐CSF has a negative effect on the osteoinductive property of this blood/BCP composite. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2830–2838, 2015.