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Composite scaffolds composed of bone marrow mesenchymal stem cell‐derived extracellular matrix and marrow clots promote marrow cell retention and proliferation
Author(s) -
Wei Bo,
Guo Yang,
Xu Yan,
Mao Fengyong,
Yao Qingqiang,
Jin Chengzhe,
Gu Qiangrong,
Wang Liming
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35373
Subject(s) - bone marrow , materials science , extracellular matrix , fibrocartilage , mesenchymal stem cell , biomedical engineering , cartilage , pathology , microbiology and biotechnology , medicine , biology , anatomy , osteoarthritis , alternative medicine , articular cartilage
Various biomaterials have been investigated in attempts to improve the mechanical stability of marrow clots derived from microfracture to obtain repaired tissue closely resembling hyaline cartilage. The goal of this study was to investigate the retention, adhesion, proliferation, and cartilage extracellular matrix (ECM) production of marrow clot‐derived cells within a bone marrow mesenchymal stem cell‐derived (BMSC‐d) ECM/marrow clot composite scaffold. We fabricated BMSC‐dECM/marrow clot composite scaffolds and kept them in chondrogenic medium in vitro for 1, 3, or 6 weeks. Unmodified marrow clots were used as a control. The BMSC‐dECM/marrow clot composite scaffold exhibited a porous structure suitable for cell attachment and growth and further maintained cell viability. The DNA content measurements revealed that more cells proliferated in the BMSC‐dECM/marrow clot composite scaffolds over time than in the marrow clots. Furthermore, the histologic, immunohistochemical, and western blot results demonstrated that the BMSC‐dECM/marrow clot composite scaffold produced more hyaline‐like cartilage and less fibrocartilage than the marrow clot in culture. Taken together, these findings indicate that the porous BMSC‐dECM/marrow clot composite scaffold promotes the retention, attachment, and proliferation of cells from the marrow clot, and thus can stabilize the marrow clot to support chondrogenesis. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2374–2382, 2015.