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Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells
Author(s) -
Ribeiro Clarisse,
Pärssinen Jenita,
Sencadas Vítor,
Correia Vítor,
Miettinen S.,
Hytönen Vesa P.,
LancerosMéndez Senentxu
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35368
Subject(s) - materials science , adipose tissue , stem cell , stimulation , piezoelectricity , biomedical engineering , microbiology and biotechnology , neuroscience , composite material , biology , medicine
This work reports on the influence of the substrate polarization of electroactive β‐poly(vinylidene fluoride) (β‐PVDF) on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β‐PVDF surfaces (non‐poled and “poled −”) adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. “Poled −” β‐PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2172–2175, 2015.