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Cholecalciferol synthesized after UV ‐activation of 7‐dehydrocholesterol onto titanium implants inhibits osteoclastogenesis in vitro
Author(s) -
Satué María,
Ramis Joana M.,
Monjo Marta
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35364
Subject(s) - materials science , osteoclast , osteoblast , in vitro , immunostaining , cell culture , resorption , titanium , biophysics , cancer research , biochemistry , biology , immunology , endocrinology , genetics , immunohistochemistry , metallurgy
UV‐activated 7‐dehydrocholesterol (7‐DHC) has been successfully used as a biocompatible coating for titanium (Ti) implants producing active vitamin D with positive effect on osteoblast differentiation. Since an osseointegrating implant must promote bone formation while delay resorption, here we determine the effect of this coating on the pre‐osteoclast cell line RAW 264.7. Moreover, D 3 synthesis was optimized by (1) the supplementation with VitE of the 7‐DHC coating to reduce 7‐DHC oxidation and (2) the addition of an incubation step (48 h at 23°C) after UV‐irradiation to favor isomerization. In vitro results with RAW264.7 cells showed no cytotoxic effect of the coatings and a significant decrease of osteoclastogenesis. Indeed, TRAP immunostaining suggested an inhibition of Trap‐positive multinucleated cells and the mRNA levels of different phenotypic, fusion, and activity markers were reduced, particularly with 7‐DHC:VitE. In conclusion, we demonstrate an improvement of the D 3 synthesis from UV‐activated 7‐DHC when combined with VitE and show that these implants inhibit osteoclastogenesis in vitro . © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2280–2288, 2015.