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Fabrication of cancellous biomimetic chitosan‐based nanocomposite scaffolds applying a combinational method for bone tissue engineering
Author(s) -
Jamalpoor Zahra,
Mirzadeh Hamid,
Joghataei Mohammad Taghi,
Zeini Darya,
BagheriKhoulenjani Shadab,
Nourani Mohammad Reza
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35320
Subject(s) - gelatin , materials science , crystallinity , fourier transform infrared spectroscopy , cancellous bone , nanocomposite , chemical engineering , scanning electron microscope , tissue engineering , chitosan , biomedical engineering , transmission electron microscopy , polymer , composite material , nanotechnology , organic chemistry , medicine , chemistry , pathology , engineering
The aim of this study was to mimic the specific structure of bone and fabricate a biomimetic nano‐hydroxyapatite (HA)/chitosan (Cs)/gelatin scaffolds using combination of particle leaching and freeze drying methods eliminating mold effects. To achieve an optimum structure, scaffolds with different gelatin/Cs weight ratio were fabricated. Morphological characterization of scaffolds by scanning electron microscopy method showed highly interconnected porous structures similar to cancellous bone with mean pore size ranging from 140 to 190 μm. Nano‐HA crystals were dispersed homogeneously in the polymer matrix according to the energy‐dispersive X‐ray spectroscopy and transmission electron microscopy images. Fourier transform infrared and X‐ray diffraction results disclosed that chemical interactions were formed between nano‐HA, Cs, gelatin and crystallinity of each material decreased with blending. It was found that increasing the gelatin content significantly improved water uptake, degradation rate as well as attachment, infiltration and proliferation of Saos2 cells to the scaffolds. The presented results confirm that the designed biomimetic nano‐HA /Cs/gelatin scaffolds can be used as promising substitutes for bone tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1882–1892, 2015.