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Porous PLGA microspheres tailored for dual delivery of biomolecules via layer‐by‐layer assembly
Author(s) -
Go Dewi P.,
Palmer Jason A.,
Mitchell Geraldine M.,
Gras Sally L.,
O'Connor Andrea J.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35319
Subject(s) - materials science , plga , biomolecule , polyelectrolyte , self healing hydrogels , nanotechnology , tissue engineering , biophysics , polymer , biomedical engineering , chemical engineering , polymer chemistry , composite material , nanoparticle , medicine , engineering , biology
Tissue engineering is a complex and dynamic process that requires varied biomolecular cues to promote optimal tissue growth. Consequently, the development of delivery systems capable of sequestering more than one biomolecule with controllable release profiles is a key step in the advancement of this field. This study develops multilayered polyelectrolyte films incorporating alpha‐melanocyte stimulating hormone (α‐MSH), an anti‐inflammatory molecule, and basic fibroblast growth factor (bFGF). The layers were successfully formed on macroporous poly lactic‐ co ‐glycolic acid microspheres produced using a combined inkjet and thermally induced phase separation technique. Release profiles could be varied by altering layer properties including the number of layers and concentrations of layering molecules. α‐MSH and bFGF were released in a sustained manner and the bioactivity of α‐MSH was shown to be preserved using an activated macrophage cell assay in vitro . The system performance was also tested in vivo subcutaneously in rats. The multilayered microspheres reduced the inflammatory response induced by a carrageenan stimulus 6 weeks after implantation compared to the non‐layered microspheres without the anti‐inflammatory and growth factors, demonstrating the potential of such multilayered constructs for the controlled delivery of bioactive molecules. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1849–1863, 2015.