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Transferrin surface‐modified PLGA nanoparticles‐mediated delivery of a proteasome inhibitor to human pancreatic cancer cells
Author(s) -
Frasco Manuela F.,
Almeida Gabriela M.,
SantosSilva Filipe,
Pereira Maria do Carmo,
Coelho Manuel A. N.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35286
Subject(s) - plga , pancreatic cancer , bortezomib , transferrin , cancer cell , cytotoxicity , internalization , materials science , drug delivery , transferrin receptor , cancer research , cytotoxic t cell , pharmacology , biophysics , cancer , in vitro , cell , chemistry , nanoparticle , medicine , nanotechnology , biochemistry , immunology , biology , multiple myeloma
The aim of this study was to develop a drug delivery system based on poly(lactic‐ co ‐glycolic acid) (PLGA) nanoparticles for an efficient and targeted action of the proteasome inhibitor bortezomib against pancreatic cancer cells. The PLGA nanoparticles were formulated with a poloxamer, and further surface‐modified with transferrin for tumor targeting. The nanoparticles were characterized as polymer carriers of bortezomib, and the cellular uptake and growth inhibitory effects were evaluated in pancreatic cells. Cellular internalization of nanoparticles was observed in normal and cancer cells, but with higher uptake by cancer cells. The sustained release of the loaded bortezomib from PLGA nanoparticles showed cytotoxic effects against pancreatic normal and cancer cells. Noteworthy differential cytotoxicity was attained by transferrin surface‐modified PLGA nanoparticles since significant cell growth inhibition by delivered bortezomib was only observed in cancer cells. These findings demonstrate that the ligand transferrin enhanced the targeted delivery of bortezomib‐loaded PLGA nanoparticles to pancreatic cancer cells. These in vitro results highlight the transferrin surface‐modified PLGA nanoparticles as a promising system for targeted delivery of anticancer drugs. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1476–1484, 2015.