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BMP‐2 encapsulated polysaccharide nanoparticle modified biphasic calcium phosphate scaffolds for bone tissue regeneration
Author(s) -
Wang Zhenming,
Wang Kefeng,
Lu Xiong,
Li Minqi,
Liu Hongrui,
Xie Chaoming,
Meng Fanzhi,
Jiang Ou,
Li Chen,
Zhi Wei
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35282
Subject(s) - materials science , regeneration (biology) , nanoparticle , calcium , bone morphogenetic protein 2 , polysaccharide , phosphate , biomedical engineering , tissue engineering , scaffold , chemical engineering , biophysics , nanotechnology , biochemistry , microbiology and biotechnology , in vitro , biology , metallurgy , medicine , engineering
Bone morphology protein‐2 (BMP‐2) encapsulated chitosan/chondrotin sulfate nanoparticles (CHI/CS NPs) are developed to enhance ectopic bone formation on biphasic calcium phosphate (BCP) scaffolds. BMP‐2 contained CHI/CS NPs were prepared by a simple and mild polyelectrolyte complexation process. It does not involve harsh organic solvents and high temperature, and therefore retain growth factors activity. These NPs were immobilized on BCP scaffolds, and realize the sustained release of growth factors from the scaffolds. The bare BCP scaffolds, NP loaded scaffolds (BCP‐NP), and NP loaded and polydopamine coated scaffolds (BCP‐Dop‐NP) were seeded with bone marrow stroma cells (BMSC) to evaluate the osteoinductivity of the scaffolds. BMSC culture results indicate that all scaffolds favor cell adhesion, proliferation, differentiation. Afterwards, the bare BCP, BCP‐NP, and BCP‐Dop‐NP scaffolds were implanted into rabbits intramuscularly to evaluate the ectopic bone formation of scaffolds. In vivo results indicate that the BCP‐NP and BCP‐Dop‐NP scaffolds enhance more ectopic bone formation than the bare BCP scaffolds. Both the in vitro and in vivo results demonstrate that BMP‐2 encapsulated polysaccharide NPs are effective to improve the osteoinductivity of the scaffolds. In addition, BCP‐NP scaffolds induce more bone formation than BCP‐Dop‐NP scaffolds. This is because BCP‐NP scaffolds harness the intrinsic osteoinductivity BCP and BMP‐2, whereas BCP‐Dop‐NP scaffolds have polydopamine coatings that inhibit the surfaces biological features of BCP scaffolds, and therefore weaken the bone formation ability of scaffolds. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1520–1532, 2015.