Premium
Development of a porcine renal extracellular matrix scaffold as a platform for kidney regeneration
Author(s) -
Choi Seock Hwan,
Chun So Young,
Chae Seon Yeong,
Kim Jin Rae,
Oh Se Heang,
Chung Sung Kwang,
Lee Jin Ho,
Song Phil Hyun,
Choi GyuSeog,
Kim TaeHwan,
Kwon Tae Gyun
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35274
Subject(s) - scaffold , extracellular matrix , decellularization , regeneration (biology) , biocompatibility , materials science , biomedical engineering , kidney , matrix (chemical analysis) , tissue engineering , sodium dodecyl sulfate , biophysics , chemistry , microbiology and biotechnology , medicine , biochemistry , biology , composite material , metallurgy
Acellular scaffolds, possessing an intact three‐dimensional extracellular matrix (ECM) architecture and biochemical components, are promising for regeneration of complex organs, such as the kidney. We have successfully developed a porcine renal acellular scaffold and analyzed its physical/biochemical characteristics, biocompatibility, and kidney reconstructive potential. Segmented porcine kidney cortexes were treated with either 1% (v/v) Triton X‐100 (Triton) or sodium dodecyl sulfate (SDS). Scanning electron microscopy showed both treatments preserved native tissue architecture, including porosity and composition. Swelling behavior was higher in the Triton‐treated compared with the SDS‐treated scaffold. Maximum compressive strength was lower in the Triton‐treated compared with the SDS‐treated scaffold. Attenuated total reflective‐infrared spectroscopy showed the presence of amide II (NH) in both scaffolds. Furthermore, richer ECM protein and growth factor contents were observed in the Triton‐treated compared with SDS‐treated scaffold. Primary human kidney cell adherence, viability, and proliferation were enhanced on the Triton‐treated scaffold compared with SDS‐treated scaffold. Following murine in vivo implantation, tumorigenecity was absent for both scaffolds after 8 weeks and in the Triton‐treated scaffold only, glomeruli‐like structure formation and neovascularity were observed. We identified 1% Triton X‐100 as a more suitable decellularizing agent for porcine renal ECM scaffolds prior to kidney regeneration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1391–1403, 2015.