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Growth, cell cycle progression, and morphology of 3T3 cells following fibroin microsphere ingestion
Author(s) -
Go Nam Kyung,
Lee Jin Sil,
Lee Joon Ho,
Hur Won
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35269
Subject(s) - fibroin , flow cytometry , materials science , biophysics , confocal microscopy , fluorescence microscope , viability assay , confocal , cell growth , 3t3 cells , cell , morphology (biology) , fluorescence , microbiology and biotechnology , cell culture , chemistry , biochemistry , biology , silk , transfection , physics , geometry , mathematics , genetics , quantum mechanics , composite material
Cellular uptake of microspheres may cause physiological stress and toxicity. In this report, we investigated the effect of cellular uptake of fibroin microspheres on the growth, cell cycle progression, and morphology of 3T3 cells. The microspheres were prepared by physical cross‐linking of fibroin molecules without any chemical modification. Fluorescent microspheres are comprised of fluorescein isothiocyanate–dextran core and fibroin shell. More than 90% of cells were determined to be fluorescence‐positive following 24‐h incubation with fluorescent microspheres (0.17 mg/mL). Microsphere localization in the cytoplasm was demonstrated using confocal and transmission electron microscopy. Cellular uptake of microspheres did not influence cellular viability, but microsphere concentrations above 0.1 mg/mL resulted in decreased cell proliferation. The proliferation inhibition was attributed to G 2 /M phase delay in cell cycle progression and S‐phase delay at higher microsphere concentrations (0.33 mg/mL). Although flow cytometry light‐scattering data raised the possibility of morphological changes, Coulter counter analysis confirmed no significant size differences between cells incubated with and without microspheres. Accordingly, fibroin microspheres can be a potential vehicle for intracytoplasmic delivery of cargos, without affecting cell viability. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1325–1331, 2015.