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TiO 2 ‐coated CoCrMo: Improving the osteogenic differentiation and adhesion of mesenchymal stem cells in vitro
Author(s) -
Logan Niall,
Sherif Anas,
Cross Alison J.,
Collins Simon N.,
Traynor Alison,
Bozec Laurent,
Parkin Ivan P.,
Brett Peter
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35264
Subject(s) - materials science , biocompatibility , titanium , chemical vapor deposition , mesenchymal stem cell , adhesion , surface modification , coating , cell adhesion , vinculin , chemical engineering , nanotechnology , composite material , metallurgy , microbiology and biotechnology , engineering , biology
The current gold standard material for orthopedic applications is titanium (Ti), however, other materials such as cobalt–chromium–molybdenum (CoCrMo) are often preferred due to their wear resistance and mechanical strength. This study investigates if the bioactivity of CoCrMo can be enhanced by coating the surface with titanium oxide (TiO 2 ) by atmospheric pressure chemical vapor deposition (CVD), thereby replicating the surface oxide layer found on Ti. CoCrMo, TiO 2 ‐coated CoCrMo (CCMT) and Ti substrates were used for this study. Cellular f‐actin distribution was shown to be noticeably different between cells on CCMT and CoCrMo after 24 h in osteogenic culture, with cells on CCMT exhibiting greater spread with developed protrusions. Osteogenic differentiation was shown to be enhanced on CCMT compared to CoCrMo, with increased calcium ion content per cell ( p < 0.05), greater hydroxyapatite nodule formation ( p < 0.05) and reduced type I collagen deposition per cell ( p < 0.05). The expression of the focal adhesion protein vinculin was shown to be marginally greater on CCMT compared to CoCrMo, whereas AFM results indicated that CCMT required more force to remove a single cell from the substrate surface compared to CoCrMo ( p < 0.0001). These data suggest that CVD TiO 2 coatings may have the potential to increase the biocompatibility of CoCrMo implantable devices. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1208–1217, 2015.