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Gelatin–siloxane nanoparticles to deliver nitric oxide for vascular cell regulation: Synthesis, cytocompatibility, and cellular responses
Author(s) -
Zhang QinYuan,
Wang ZuYong,
Wen Feng,
Ren Lei,
Li Jun,
Teoh Swee Hin,
Thian Eng San
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35239
Subject(s) - enos , nitric oxide , gelatin , materials science , restenosis , cell growth , siloxane , cell , nanoparticle , vascular smooth muscle , umbilical vein , biophysics , microbiology and biotechnology , nanotechnology , biochemistry , chemistry , nitric oxide synthase , medicine , biology , smooth muscle , organic chemistry , in vitro , stent , composite material , polymer
Nitric oxide (NO) is an important mediator in cardiovascular system to regulate vascular tone and maintain tissue homeostasis. Its role in vascular cell regulation makes it promising to address the post‐surgery restenosis problem. However, the application of NO is constrained by its high reactivity. Here, we developed a novel NO‐releasing gelatin–siloxane nanoparticle (GS–NO NP) to deliver NO effectively for vascular cell regulation. Results showed that gelatin–siloxane nanoparticles (GS NPs) could be synthesized via sol‐gel chemistry with a diameter of ∼200 nm. It could be modified into GS–NO NPs via S ‐nitrosothiol (RSNO) modification. The synthesized GS–NO NPs could release a total of ∼0.12 µmol/mg NO sustainably for 7 days following a first‐order exponential profile. They showed not only excellent cytocompatibility, but also rapid intracellularization within 2 h. GS–NO NPs showed inhibition of human aortic smooth muscle cell (AoSMC) proliferation and promotion of human umbilical vein endothelial cell (HUVEC) proliferation in a dose‐dependent manner, which is an important approach to prevent restenosis. With GS–NO NP dose at 100 µg/mL, the proliferation of AoSMCs could be slowed down whereas the growth of HUVECs was significantly promoted. We concluded that GS–NO NPs could have potential to be used as a promising nano‐system to deliver NO for vascular cell regulation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 929–938, 2015.