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Comparison of surface modification chemistries in mouse, porcine, and human islets
Author(s) -
SoRelle Jeffrey A.,
Kanak Mazhar A.,
Itoh Takeshi,
Horton Joshua M.,
Naziruddin Bashoo,
Kane Robert R.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35229
Subject(s) - islet , biocompatibility , transplantation , surface modification , pancreatic islets , covalent bond , materials science , immune system , biophysics , biochemistry , biomedical engineering , chemistry , immunology , medicine , diabetes mellitus , biology , endocrinology , organic chemistry , metallurgy
Beta cell replacement therapy, the transplantation of isolated pancreatic islets by intraportal infusion, offers patients with brittle type 1 diabetes blood glucose regulation with a minimally invasive technique. Chemical modification of islets prior to transplantation, providing a nanothin barrier that potentially includes active protective compounds, has been proposed as a strategy to minimize the inflammatory and immune reactions that often significantly limit graft function and duration. Chemical modification also has the potential to allow the use of alternative sources of islets, such as porcine islets, for transplantation. This investigation compared three orthogonal covalent islet modification techniques across three species (human, porcine, and murine), using multiple measures to determine biocompatibility and effectiveness. All three conjugation chemistries were well tolerated, and the overall efficiency, gross uniformity, and stability of the surface modifications were dependent upon the conjugation chemistry as well as the islet source (human, porcine, or murine). Notably, the reductive modification of surface disulfides was shown to afford intense and long‐lasting modification of human islets. This study demonstrates that murine, human, and porcine islets tolerate a variety of covalent modifications, that these modifications are relatively stable, and that the murine islet model may not be predictive for some chemical contexts. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 869–877, 2015.