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Osteochondral regeneration using an oriented nanofiber yarn‐collagen type I/hyaluronate hybrid/TCP biphasic scaffold
Author(s) -
Liu Shen,
Wu Jinglei,
Liu Xudong,
Chen Desheng,
Bowlin Gary L.,
Cao Lei,
Lu Jianxi,
Li Fengfeng,
Mo Xiumei,
Fan Cunyi
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35206
Subject(s) - materials science , scaffold , biomedical engineering , regeneration (biology) , tissue engineering , cartilage , electrospinning , nanofiber , compressive strength , composite material , polymer , anatomy , microbiology and biotechnology , medicine , biology
Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge. Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength. In this study, an oriented poly( l ‐lacticacid)‐ co ‐poly( ε ‐caprolactone) P(LLA‐CL)/collagen type I(Col‐I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze‐dried Col‐I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold. In vitro results show that the Yarn Col‐I/HA hybrid scaffold (Yarn‐CH) can allow the cell infiltration like sponge scaffolds. Using porous beta‐tricalcium phosphate (TCP) as the osseous phase, the Yarn‐CH/TCP biphasic scaffold was then assembled by freeze drying. After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 581–592, 2015.