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Real time visualization and characterization of platelet deposition under flow onto clinically relevant opaque surfaces
Author(s) -
Jamiolkowski Megan A.,
Woolley Joshua R.,
Kameneva Marina V.,
Antaki James F.,
Wagner William R.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35202
Subject(s) - materials science , opacity , characterization (materials science) , visualization , deposition (geology) , flow visualization , flow (mathematics) , nanotechnology , biomedical engineering , mechanics , optics , artificial intelligence , computer science , medicine , paleontology , physics , sediment , biology
Although the thrombogenic nature of the surfaces of cardiovascular devices is an important aspect of blood biocompatibility, few studies have examined platelet deposition onto opaque materials used for these devices in real time. This is particularly true for the metallic surfaces used in current ventricular assist devices (VADs). Using hemoglobin depleted red blood cells (RBC ghosts) and long working distance optics to visualize platelet deposition, we sought to perform such an evaluation. Fluorescently labeled platelets mixed with human RBC ghosts were perfused across six opaque materials (a titanium alloy (Ti6Al4V), silicon carbide (SiC), alumina (Al 2 O 3 ), 2‐methacryloyloxyethyl phosphorylcholine polymer coated Ti6Al4V (MPC‐Ti6Al4V), yttria partially stabilized zirconia (YZTP), and zirconia toughened alumina (ZTA)) for 5 min at wall shear rates of 400 and 1000 s −1 . Ti6Al4V had significantly increased platelet deposition relative to MPC‐Ti6Al4V, Al 2 O 3 , YZTP, and ZTA at both wall shear rates ( p < 0.01). For all test surfaces, increasing the wall shear rate produced a trend of decreased platelet adhesion. The described system can be a utilized as a tool for comparative analysis of candidate blood‐contacting materials with acute blood contact. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1303–1311, 2015.