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In vitro age dependent response of macrophages to micro and nano titanium dioxide particles
Author(s) -
Bruno Marcos E.,
Sittner Maximiliano,
Cabrini Rómulo L.,
Guglielmotti María B.,
Olmedo Daniel G.,
Tasat Deborah R.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35194
Subject(s) - materials science , in vitro , nitric oxide , superoxide , titanium dioxide , cytokine , biophysics , particle size , tumor necrosis factor alpha , nanoparticle , immunology , nanotechnology , biology , biochemistry , composite material , endocrinology , enzyme , paleontology
As a result of corrosion, microparticles (MP) and/or nanoparticles (NP) can be released from the metallic implants surface into the bioenvironment. The biological response to these particles depends not only on the physico‐chemical properties of the particles but also on host factors, such as age. Macrophages have attracted wide concern in biomedicine. The aim of this investigation was to study the age related biological response of macrophages to TiO 2 ‐MP and NP in vitro . Alveolar macrophages (AM) obtained from young and senescent rats were cultured and exposed to TiO 2 ‐MP and NP. Cell metabolism, superoxide anion (O 2 − ) and nitric oxide (NO) generation, and cytokine release (IL‐6, TNFα, IL‐10) were measured. Cell metabolism was not affected by particle exposure. O 2 − and NO generation increased in a dose dependent manner. A marked increase on IL‐6 release was found in the young‐AM subpopulation exposed to TiO 2 ‐MP. Conversely, both particle sizes induced a dose dependent release of TNFα in senescent‐AM. Only the highest concentration of TiO 2 ‐particles caused a significant increase in IL‐10 release in AM‐cultures. These observations lend strong support to the suggestion that cellular response of macrophages to TiO 2 ‐particles is age dependent. The biological effect of the particles would seem to be more deleterious in the senescent age‐group. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 471–478, 2015.