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Different influence of Ti, PMMA, UHMWPE, and Co‐Cr particles on peripheral blood monocytes during periprosthetic inflammation
Author(s) -
Zhang Kai,
Yang ShangYou,
Yang Shuye,
Bai Ling,
Li Peng,
Liu Dong,
Schurman John R.,
Wooley Paul H.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35176
Subject(s) - peripheral blood mononuclear cell , periprosthetic , proinflammatory cytokine , chemokine , materials science , monocyte , inflammation , tumor necrosis factor alpha , biomaterial , immunology , pathology , microbiology and biotechnology , medicine , in vitro , biology , arthroplasty , surgery , biochemistry , nanotechnology
This study investigated cellular trafficking and inflammatory markers in orthopedic biomaterial particle‐challenged human peripheral blood monocytes (PBMCs) using a murine immunodeficiency (SCID) model. Periprosthetic tissues from aseptic loosening patients were transplanted into muscles of SCID mice. PBMCs from the same patients were stimulated in vitro with Ti‐6Al‐4V, PMMA, UHMWPE, or Co‐Cr particles for 3 days before administered intraperitoneally to the periprosthetic tissue‐implanted mice. The xenografts were harvested 2 weeks later for histological and molecular analyses. Significant cell infiltration was obvious in the transplanted tissues from mice transfused with Ti‐alloy, PMMA and UHMWPE‐provoked PBMCs compared to controls, and UHMWPE‐provoked PBMCs group accumulated significantly more cells among all groups. There were ubiquitous TRAP+ stained cells in all xenografts from particle‐stimulated PBMCs mice. Immunohistochemical staining indicated that significantly more IL‐1β and TNF positive cells occurred in Ti and PMMA groups; while the UHMWPE group resulted in stronger positive MCP‐1 and IL‐6 stains. Polymerase chain reaction (PCR) confirmed overexpression of both IL‐1β and TNF in Ti and PMMA‐stimulated groups; and more MIP‐1α gene expression developed in the UHMWPE group. Overall, different type of orthopedic materials influenced the trafficking ability of particle‐activated PBMCs which may depend on upregulation of various proinflammatory cytokines and chemokines. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 358–364, 2015.