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Osteoregenerative capacities of dicalcium phosphate‐rich calcium phosphate bone cement
Author(s) -
Ko ChiaLing,
Chen JianChih,
Tien YinChun,
Hung ChunCheng,
Wang JenChyan,
Chen WenCheng
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35167
Subject(s) - materials science , alkaline phosphatase , calcium , viability assay , bone cement , biomedical engineering , phosphate , nuclear chemistry , apatite , cement , dentistry , in vitro , chemistry , mineralogy , composite material , biochemistry , medicine , enzyme , metallurgy
Calcium phosphate cement (CPC) is a widely used bone substitute. However, CPC application is limited by poor bioresorption, which is attributed to apatite, the stable product. This study aims to systematically survey the biological performance of dicalcium phosphate (DCP)‐rich CPC. DCP‐rich CPC exhibited a twofold, surface‐modified DCP anhydrous (DCPA)‐to‐tetracalcium phosphate (TTCP) molar ratio, whereas conventional CPC (c‐CPC) showed a onefold, surface unmodified DCPA‐to‐TTCP molar ratio. Cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity in the two CPCs were examined with bone cell progenitor D1 cultured in vitro . Microcomputed tomography and histological observation were conducted after CPC implantation in vivo to analyze the residual implant ratio and new bone formation rate. D1 cells cultured on DCP‐rich CPC surfaces exhibited higher cell viability, ALP activity, and ALP quantity than c‐CPC. Histological evaluation indicated that DCP‐rich CPC showed lesser residual implant and higher new bone formation rate than c‐CPC. Therefore, DCP‐rich CPC can improve bioresorption. The newly developed DCP‐rich CPC exhibited potential therapeutic applications for bone reconstruction. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 203–210, 2015.