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Exposure of polyethylene particles induces interferon‐γ expression in a natural killer T lymphocyte and dendritic cell coculture system in vitro : A preliminary study
Author(s) -
Lin TzuHua,
Kao Sunny,
Sato Taishi,
Pajarinen Jukka,
Zhang Ruth,
Loi Florence,
Goodman Stuart B.,
Yao Zhenyu
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35159
Subject(s) - natural killer t cell , chemokine , osteolysis , immunology , tumor necrosis factor alpha , interferon , microbiology and biotechnology , cytokine , macrophage , materials science , inflammation , cancer research , biology , t cell , in vitro , immune system , medicine , biochemistry , surgery
Two major issues in total joint arthroplasty are loosening of implants and osteolysis caused by wear particle‐induced inflammation. Wear particles stimulate the release of pro‐inflammatory cytokines, chemokines, and other inflammatory mediators from macrophages and other cells. Although the biological response of macrophages to wear debris is well established, the role of other cell types such as natural killer T lymphocytes (NKT) and dendritic cells (DCs) is limited. Here we show that ultra‐high molecular weight polyethylene (UHMWPE) particles stimulate NKT cells to secrete Interferon‐γ (IFN‐γ); coculture with DCs further enhanced IFN‐γ secretion. Furthermore, UHMWPE particles did not stimulate NKT cells to secrete IL‐4, while the NKT cell natural ligand α‐galactosylceramide (α‐GalCer) treatment in the coculture system significantly enhanced both IFN‐γ and IL‐4 expression by NKT cells. Comparatively, NKT cells and/or DCs exposed to polymethylmethacrylate particles did not stimulate IFN‐γ or IL‐4 expression. Mouse bone marrow derived macrophage polarization by lipopolysaccharide and conditioned medium from NKT cells and/or DCs exposed to UHMWPE particles increased tumor necrosis factor‐α (TNF‐α), but reduced arginase‐1 expression in macrophages. The current findings indicate that UHMWPE particles stimulate NKT cells/DCs to produce pro‐inflammatory cytokines; this pathway is a novel therapeutic target to mitigate wear particle induced peri‐prosthetic osteolysis. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 71–75, 2015.

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