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Sustained PDGF‐BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model
Author(s) -
Dong CuiLing,
Webb William R.,
Peng Qiang,
Tang James Z.,
Forsyth Nicholas R.,
Chen GuoQiang,
El Haj Alicia J.
Publication year - 2015
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35149
Subject(s) - materials science , mesenchymal stem cell , platelet derived growth factor receptor , growth factor , tissue engineering , cell growth , biomedical engineering , stem cell , microbiology and biotechnology , biophysics , chemistry , biology , biochemistry , receptor , medicine
This study aimed to design a growth factor loaded copolyester of 3‐hydroxybutyrate and 3‐hydroxyhexanoate (PHBHHx) nanoparticles containing 3D collagen matrix to achieve growth factor sustained release for long‐term stimulation of human mesenchymal stem cells (hMSCs) proliferation/differentiation for tissue engineer application. Platelet‐derived growth factor‐BB (PDGF‐BB), which is known to enhance hMSCs proliferation in human serum, was selected as a model growth factor, and biodegradable copolyester of PHBHHx was chosen to be the sustained release vehicle. PDGF‐BB phospholipid complex encapsulated PHBHHx nanoparticles were fabricated, and their effect on hMSCs proliferation was investigated via assays of CCK‐8 and live‐dead staining to cells inoculated in 2D tissue culture plates and 3D collagen gel scaffolds, respectively. The resulting spherical PHBHHx nanoparticles were stable in terms of their mean particle size, polydispersity index and zeta potential before and after lyophilization. In vitro study revealed a sustained release of PDGF‐BB with a low burst release. Furthermore, sustained released PDGF‐BB was revealed to significantly promote hMSCs proliferation in both cell monolayer and cell seeded 3D collagen scaffolds inoculated in serum‐free media. Therefore, the 3D collagen matrices with locally sustained release growth factor nanoparticles hold promise to be used for stem cell tissue engineering. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 282–288, 2015.