Endothelialization of electrospun polycaprolactone (PCL) small caliber vascular grafts spun from different polymer blends | Zendy

Pfeiffer D. | Zendy; Stefanitsch C. | Zendy; Wankhammer K. | Zendy; Müller M. | Zendy; Dreyer L. | Zendy; Krolitzki B. | Zendy; Zernetsch H. | Zendy; Glasmacher B. | Zendy; Lindner C. | Zendy; Lass A. | Zendy; Schwarz M. | Zendy; Muckenauer W. | Zendy; Lang I. | Zendy

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Endothelialization of electrospun polycaprolactone (PCL) small caliber vascular grafts spun from different polymer blends

Author(s) -

Pfeiffer D.,

Stefanitsch C.,

Wankhammer K.,

Müller M.,

Dreyer L.,

Krolitzki B.,

Zernetsch H.,

Glasmacher B.,

Lindner C.,

Lass A.,

Schwarz M.,

Muckenauer W.,

Lang I.

Publication year - 2014

Publication title -

journal of biomedical materials research part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.849

H-Index - 150

eISSN - 1552-4965

pISSN - 1549-3296

DOI - 10.1002/jbm.a.35123

Subject(s) - polycaprolactone , materials science , fibronectin , biomedical engineering , electrospinning , peg ratio , tissue engineering , polymer , extracellular matrix , medicine , microbiology and biotechnology , composite material , biology , finance , economics

Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre‐coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre‐coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin‐coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78–81% higher than on fibronectin pre‐treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin‐coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4500–4509, 2014.

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