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Critical size defect regeneration by rhPTH‐collagen membrane as a new tissue engineering tool
Author(s) -
Missana Liliana R.,
Jammal María V.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35114
Subject(s) - soft tissue , biomaterial , teriparatide , bone healing , biomedical engineering , regeneration (biology) , materials science , tissue engineering , hydroxyproline , medicine , anatomy , osteoporosis , surgery , endocrinology , biology , bone mineral , microbiology and biotechnology
Recombinant Human Parathyroid Hormone (rhPTH 1–34) administration is an effective treatment to improve bone mass in osteoporosis. The aim of this study was to develop a Tissue Engeenering Tool for bone regeneration. We evaluated the efficacy of a freeze dried rhPTH membrane in calvarial critical size defect (CSD). Forty‐four Wistar female rats (body weight 150 ± 50 g) with CSD (5 mm) were divided into four groups: group 1: rhPTH membrane (rhPTHm); group 2: atelocollagen membrane (Cm); group 3: rhPTH and atelocollagen I (CrhPTHm); group 4: without any treatment (CG). All samples were evaluated on the 1st, 3rd, and 6th weeks (weeks) post‐surgery by soft X‐ray, histological and histometric studies. Soft X‐ray results showed a radiolucent image with many irregular radiopaque areas. Histologically, rhPTHm was replaced by reticular bone (7%) since 3rd week, and lamellar bone ossicles (30%) at 6th week. Cm showed bone formation like composite bone type on week 1st, 3rd, and 6th (2%, 44%, and 41%, respectively). With CrhPTHm, bone formation was observed in all periods (2.4%, 48%, and 53%), showing statistical difference with CG in the 3rd and 6th wks ( p = 0.03 and 0.01). Our results demonstrated the effectiveness of a new biomaterial called CrhPTHm because its ability to regenerate calvarial CSD. Moreover, the membrane represents a new local intermittent delivery system allowing rhPTH slow release. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4358–4364, 2014.