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Tunable swelling of polyelectrolyte multilayers in cell culture media for modulating NIH‐3T3 cells adhesion
Author(s) -
Qi Wei,
Cai Peng,
Yuan Wenjing,
Wang Hua
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35094
Subject(s) - swelling , polyelectrolyte , materials science , adhesion , cell adhesion , nanotechnology , chemical engineering , biophysics , cell culture , salt (chemistry) , tissue engineering , focal adhesion , cell , biomedical engineering , polymer , chemistry , composite material , biochemistry , organic chemistry , medicine , genetics , engineering , biology
Abstract For polyelectrolyte multilayers (PEMs) assembled by the layer‐by‐layer (LbL) assembly technique, their nanostructure and properties can be governed by many parameters during the building process. Here, it was demonstrated that the swelling of the PEMs containing poly(diallyldimethylammonium chloride) (PDDA) and poly(sodium 4‐styrenesulfonate) (PSS) in cell culture media could be tuned with changing supporting salt solutions during the assembly process. Importantly, the influence of the PEMs assembled in different salt solutions on NIH‐3T3 cell adhesion was observable. Specifically, the cells could possess a higher affinity for the films assembled in low salt concentration (i.e. 0.15 M NaCl) or no salt, the poorly swelling films in cell culture media, which was manifested by the large cell spreading area and focal adhesions. In contrast, those were assembled in higher salt concentration, highly swelling films in cell culture media, were less attractive for the fibroblasts. As a result, the cell adhesion behaviors may be manipulated by tailoring the physicochemical properties of the films, which could be performed by changing the assembly conditions such as supporting salt concentration. Such a finding might promise a great potential in designing desired biomaterials for tissue engineering and regenerative medicine. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4071–4077, 2014.