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Tracheal reconstruction using chondrocytes seeded on a poly( l ‐lactic‐ co ‐glycolic acid)–fibrin/hyaluronan
Author(s) -
Hong Hyun Jun,
Chang Jae Won,
Park JuKyeong,
Choi Jae Won,
Kim Yoo Suk,
Shin Yoo Seob,
Kim ChulHo,
Choi Eun Chang
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35091
Subject(s) - hyaluronic acid , fibrin , tissue engineering , plga , tracheal stenosis , biomedical engineering , scaffold , glycolic acid , materials science , granulation tissue , respiratory epithelium , epithelium , anatomy , wound healing , pathology , airway , lactic acid , medicine , biology , surgery , nanotechnology , immunology , genetics , nanoparticle , bacteria
Reconstruction of trachea is still a clinical dilemma. Tissue engineering is a recent and promising concept to resolve this problem. This study evaluated the feasibility of allogeneic chondrocytes cultured with fibrin/hyaluronic acid (HA) hydrogel and degradable porous poly( l ‐lactic‐ co ‐glycolic acid) (PLGA) scaffold for partial tracheal reconstruction. Chondrocytes from rabbit articular cartilage were expanded and cultured with fibrin/HA hydrogel and injected into a 5 × 10 mm‐sized, curved patch‐shape PLGA scaffold. After 4 weeks in vitro culture, the scaffold was implanted on a tracheal defect in eight rabbits. Six and 10 weeks postoperatively, the implanted sites were evaluated by bronchoscope and radiologic and histologic analyses. Ciliary beat frequency (CBF) of regenerated epithelium was also evaluated. None of the eight rabbits showed any sign of respiratory distress. Bronchoscopic examination did not reveal stenosis of the reconstructed trachea and the defects were completely recovered with respiratory epithelium. Computed tomography scan showed good luminal contour of trachea. Histologic data showed that the implanted chondrocytes successfully formed neocartilage with minimal granulation tissue. CBF of regenerated epithelium was similar to that of normal epithelium. Partial tracheal defect was successfully reconstructed anatomically and functionally using allogeneic chondrocytes cultured with PLGA‐fibrin/HA composite scaffold. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4142–4150, 2014.