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The synergistic effects of the RGD density and the microenvironment on the behavior of encapsulated cells: In vitro and in vivo direct comparative study
Author(s) -
Santos Edorta,
Garate Ane,
Pedraz José Luis,
Orive Gorka,
Hernández Rosa María
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35073
Subject(s) - in vivo , in vitro , materials science , biophysics , biomedical engineering , tripeptide , cell , microbiology and biotechnology , chemistry , biochemistry , peptide , biology , medicine
The inclusion of the tripeptide RGD (Arg‐Gly‐Asp) in otherwise inert biomaterials employed for cell encapsulation has been observed to be an effective strategy to provide the immobilized cells with a more suitable microenvironment. However, some controversial results collected during the last years, especially in vivo , have questioned its effectiveness. Here, we have studied the behavior of C 2 C 12 myoblasts immobilized in alginate‐poly‐ l ‐lysine‐alginate microcapsules with different densities of RGD. The use of these microcapsules offer the advantage of avoiding native proteins influence permitting to establish direct comparisons between in vitro and in vivo assays. The results suggest that RGD‐modified matrices provide higher dynamism, achieving therapeutically more active biosystems not only in vitro , but also in vivo . The highest functionality of the immobilized cells in vitro was obtained with the lowest RGD density. However, higher RGD densities were required in vivo to obtain the same effects observed in vitro . Altogether, these results suggest the lack of in vitro–in vivo correlation when cell behavior is evaluated within different RGD‐tailored cell‐loaded scaffolds. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3965–3972, 2014.