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The morphology, proliferation rate, and population doubling time factor of adipose‐derived mesenchymal stem cells cultured on to non‐aqueous SiO 2 , TiO 2 , and hybrid sol‐gel‐derived oxide coatings
Author(s) -
Marycz Krzysztof,
KrzakRoś Justyna,
DoneszSikorska Anna,
Śmieszek Agnieszka
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35072
Subject(s) - mesenchymal stem cell , stem cell , materials science , adipose tissue , regenerative medicine , doubling time , tissue engineering , population , microbiology and biotechnology , biomedical engineering , biology , in vitro , biochemistry , medicine , environmental health
In recent years, much attention has been paid to the development of tissue engineering and regenerative medicine, especially when stem cells of various sources are concerned. In addition to the interest in mesenchymal stem cells isolated from bone marrow, recently more consideration has been given to stem cells isolated from adipose tissue (AdMSCs), due to their less invasive method of collection as well as their ease of isolation and culture. However, the development of regenerative medicine requires both the application of biocompatible material and the stem cells to accelerate the regeneration. In this study, we investigated the morphology, proliferation rate index (PRi), and population doubling time factor of adipose‐derived mesenchymal stem cells cultured on non‐aqueous sol‐gel‐derived SiO 2 , TiO 2 , and SiO 2 /TiO 2 oxide coatings. The results indicated an increase in PRi of AdMSCs when cultured on to titanium dioxide, suggesting its high attractiveness for AdMSCs. In addition, the proper morphology and the shortest doubling time of AdMSCs were observed when cultured on titanium dioxide coating. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4017–4026, 2014.