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Biocompatibility and enhanced osteogenic differentiation of human mesenchymal stem cells in response to surface engineered poly( d , l ‐lactic‐ co ‐glycolic acid) microparticles
Author(s) -
Rogers Catherine M.,
Deehan David J.,
Knuth Callie A.,
Rose Felicity R. A. J.,
Shakesheff Kevin M.,
Oldershaw Rachel A.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35063
Subject(s) - plga , biomaterial , biocompatibility , glycolic acid , materials science , biomedical engineering , mesenchymal stem cell , tissue engineering , lactic acid , nanotechnology , microbiology and biotechnology , medicine , nanoparticle , biology , bacteria , metallurgy , genetics
Tissue engineering strategies can be applied to enhancing osseous integration of soft tissue grafts during ligament reconstruction. Ligament rupture results in a hemarthrosis, an acute intra‐articular bleed rich in osteogenic human mesenchymal stem cells (hMSCs). With the aim of identifying an appropriate biomaterial with which to combine hemarthrosis fluid‐derived hMSCs (HF‐hMSCs) for therapeutic application, this work has investigated the biocompatibility of microparticles manufactured from two forms of poly( d , l ‐lactic‐ co ‐glycolic acid) (PLGA), one synthesized with equal monomeric ratios of lactic acid to glycolic acid (PLGA 50:50) and the other with a higher proportion of lactic acid (PLGA 85:15) which confers a longer biodegradation time. The surfaces of both types of microparticles were functionalized by plasma polymerization with allylamine to increase hydrophilicity and promote cell attachment. HF‐hMSCs attached to and spread along the surface of both forms of PLGA microparticle. The osteogenic response of HF‐hMSCs was enhanced when cultured with PLGA compared with control cultures differentiated on tissue culture plastic and this was independent of the type of polymer used. We have demonstrated that surface engineered PLGA microparticles are an appropriate biomaterial for combining with HF‐hMSCs and the selection of PLGA is relevant only when considering the biodegradation time for each biomedical application. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3872–3882, 2014.