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Ultrasound‐assisted fabrication of a biocompatible magnetic hydroxyapatite
Author(s) -
Zhou Gang,
Song Wei,
Hou Yongzhao,
Li Qing,
Deng Xuliang,
Fan Yubo
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35043
Subject(s) - biocompatibility , materials science , fourier transform infrared spectroscopy , transmission electron microscopy , nuclear chemistry , mtt assay , nanoparticle , nanotechnology , chemical engineering , cell growth , chemistry , metallurgy , biochemistry , engineering
This work describes the fabrication and characterization of a biocompatible magnetic hydroxyapatite (HA) using an ultrasound‐assisted co‐precipitation method. X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FT‐IR), and transmission electron microscopy (TEM) were used to characterize the structure and chemical composition of the produced samples. The M–H loops of synthesized materials were traced using a vibrating sample magnetometer (VSM) and the biocompatibility was evaluated by cell culture and MTT (3‐(4,5‐dimethylthiazol‐2‐yl)−2,5‐diphenyltetrazolium bromide) assay. Furthermore, in vivo histopathological examinations were used to evaluate the potential toxicological effects of Fe 3 O 4 ‐HA composites on kidney of SD rats injected intraperitoneally with Fe 3 O 4 ‐HA particles. The results showed that magnetic iron oxide particles first replace OH ions of HA, which are parallel to the c axis, and then enter the HA crystal lattice which produces changes in the crystal surface of HA. Chemical bond interaction was observed between PO 4 3− groups of HA and iron ions of Fe 3 O 4 . The saturation magnetization (M S ) of Fe 3 O 4 ‐HA composites was 46.36 emu/g obtained from VSM data. Cell culture and MTT assays indicated that HA could affect the growth and proliferation of HEK‐293 cells. This Fe 3 O 4 ‐HA composite produced no negative effects on cell morphology, viability, and proliferation and exhibited remarkable biocompatibility. Moreover, no inflammatory cell infiltration was observed in kidney histopathology slices. Therefore, this study succeeds to develop a Fe 3 O 4 ‐HA composite as a prospective biomagnetic material for future applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3704–3712, 2014.