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Long‐lasting in vivo and in vitro antibacterial ability of nanostructured titania coating incorporated with silver nanoparticles
Author(s) -
Cheng Hao,
Li Yong,
Huo Kaifu,
Gao Biao,
Xiong Wei
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35019
Subject(s) - in vivo , materials science , titanium , antibacterial activity , coating , biofilm , silver nanoparticle , antimicrobial , in vitro , staphylococcus aureus , implant , nanoparticle , biomedical engineering , nuclear chemistry , nanotechnology , microbiology and biotechnology , bacteria , surgery , metallurgy , chemistry , biology , medicine , biochemistry , genetics
Although titanium (Ti) implants are widely used clinically, implant‐associated bacterial infection is still one of the most serious complications in orthopedic surgery. Long‐term antibacterial properties and the ability to inhibit biofilm formation are highly desirable to prevent implant associated infection. In this study, a controllable amount of silver (Ag) nanoparticles was incorporated into titanium oxide; or titanium, nanotubes (TiO 2 ‐NTs). The reliable release and long‐term antibacterial function of Ag, in vivo and in vitro , and influence normal bone‐implant integration from the Ag released from Ag‐incorporated NTs in vivo have been studied to make them useable in clinical practice. In the current study, TiO 2 ‐NTs loaded with Ag (NT‐Ag) exhibited strong antibacterial activity against methicillin‐resistant Staphylococcus aureus (MRSA, ATCC43300) in vitro for 30 days, and the ability to penetrate the protein layer well. In addition, X‐ray examination and 2‐[ 18 F]‐fiuoro‐2‐deoxy‐D‐glucose positron emission tomography indicates that NT‐Ag show extremely long antibacterial activity in vivo in a rat model. Furthermore, histomorphometric analysis demonstrated that satisfactory bio‐integration can be expected. Our results indicate that NT‐Ag has both simultaneous antimicrobial and excellent bio‐integration properties, make it a promising therapeutic material for orthopedic application. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3488–3499, 2014.