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Oxidized alginate hydrogels as niche environments for corneal epithelial cells
Author(s) -
Wright Bernice,
De Bank Paul A.,
Luetchford Kim A.,
Acosta Fernando R.,
Con Che J.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35011
Subject(s) - self healing hydrogels , extracellular matrix , viability assay , biophysics , matrix (chemical analysis) , tissue engineering , materials science , cell , cornea , extracellular , cell encapsulation , wound healing , microbiology and biotechnology , regeneration (biology) , biomedical engineering , chemistry , biochemistry , polymer chemistry , biology , immunology , ophthalmology , medicine , composite material
Chemical and biochemical modification of hydrogels is one strategy to create physiological constructs that maintain cell function. The aim of this study was to apply oxidised alginate hydrogels as a basis for development of a biomimetic niche for limbal epithelial stem cells that may be applied to treating corneal dysfunction. The stem phenotype of bovine limbal epithelial cells (LEC) and the viability of corneal epithelial cells (CEC) were examined in oxidised alginate gels containing collagen IV over a 3‐day culture period. Oxidation increased cell viability ( P ≤ 0.05) and this improved further with addition of collagen IV ( P ≤ 0.01). Oxidised gels presented larger internal pores (diameter: 0.2–0.8 µm) than unmodified gels (pore diameter: 0.05–0.1 µm) and were significantly less stiff ( P ≤ 0.001), indicating that an increase in pore size and a decrease in stiffness contributed to improved cell viability. The diffusion of collagen IV from oxidised alginate gels was similar to that of unmodified gels suggesting that oxidation may not affect the retention of extracellular matrix proteins in alginate gels. These data demonstrate that oxidised alginate gels containing corneal extracellular matrix proteins can influence corneal epithelial cell function in a manner that may impact beneficially on corneal wound healing therapy. © 2013 The Authors. Journal of Biomedical Materials Research Part A Published byWiley Periodicals, Inc. Part A: 102A: 3393–3400, 2014.