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Development of polycaprolactone porous scaffolds by combining solvent casting, particulate leaching, and polymer leaching techniques for bone tissue engineering
Author(s) -
Thadavirul Napapaphat,
Pavasant Prasit,
Supaphol Pitt
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.35010
Subject(s) - materials science , polycaprolactone , interconnectivity , polyethylene glycol , leaching (pedology) , porosity , chemical engineering , polymer , biomedical engineering , solvent , peg ratio , scaffold , absorption of water , tissue engineering , composite material , organic chemistry , chemistry , medicine , environmental science , finance , artificial intelligence , computer science , soil science , engineering , economics , soil water
Sodium chloride and polyethylene glycol (PEG) were used as water‐soluble porogens for the formation of porous polycaprolactone (PCL) scaffolds. The main purpose was to prepare and evaluate in vitro efficacy of highly interconnected, three‐dimensional, porous polymeric scaffolds, as obtained from the combined particulate and polymer leaching techniques. Microscopic analysis confirmed the high interconnectivity of the pores and relatively uniform pore size of 378–435 μm. The PCL scaffolds were further characterized for their density and pore characteristics, water absorption and flow behaviors, and mechanical properties and the potential for their use as bone scaffolding materials was evaluated in vitro using mouse calvaria‐derived preosteoblastic cells (MC3T3‐E1). Evidently, the use of PEG as the secondary porogen not only improved the interconnectivity of the pore structures but also resulted in the PCL scaffolds that exhibited much better support for the proliferation and differentiation of the cultured bone cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3379–3392, 2014.