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The effect of graphene substrate on osteoblast cell adhesion and proliferation
Author(s) -
Aryaei Ashkan,
Jayatissa Ahalapitiya H.,
Jayasuriya Ambalangodage C.
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34993
Subject(s) - graphene , materials science , substrate (aquarium) , raman spectroscopy , graphene oxide paper , wafer , chemical vapor deposition , adhesion , graphene foam , graphene nanoribbons , nanotechnology , biocompatibility , osteoblast , silicon , chemical engineering , composite material , optoelectronics , metallurgy , chemistry , optics , oceanography , physics , engineering , geology , in vitro , biochemistry
Understanding the effect of graphene substrate on graphene–cell interaction is important for considering graphene as a potential candidate for biomedical applications. In this article, biocompatibility of few layers of graphene film transferred to different substrates was evaluated using osteoblasts. The substrates were oxidized silicon wafer (SiO 2 /Si stack), soda lime glass, and stainless steel. Chemical vapor deposition method was employed to synthesize graphene on copper substrate using methane and hydrogen as precursors. The quality and the thickness of graphene films on different substrates were estimated by Raman spectra, whereas the thickness of graphene film was confirmed by reflectance and transmittance spectroscopy. The study was also focused on cell attachment and morphology at two time points. The results show that graphene does not have any toxic effect on osteoblasts. The cell adhesion improves with graphene coated substrate than the substrate alone. It seems that graphene substrate properties play a dominant role in cell adhesion. The result of this study suggests that a layer of graphene on bone implants will be beneficial for osteoblast attachment and proliferation. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3282–3290, 2014.