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Gene expression profile study on osteoinductive effect of natural hydroxyapatite
Author(s) -
Lü Xiaoying,
Wang Jiandan,
Li Bin,
Zhang Zhiwei,
Zhao Lifeng
Publication year - 2014
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.34951
Subject(s) - wnt signaling pathway , gene , microarray , gene expression , bone morphogenetic protein 2 , biology , microarray analysis techniques , mesenchymal stem cell , microbiology and biotechnology , computational biology , genetics , in vitro
The aim of this study was to investigate the osteoinductive effect of natural hydroxyapatite (NHA). NHA was extracted from pig bones and prepared into disk‐like samples. Then, proliferation of mouse bone mesenchymal stem cells (MSCs) cultured on NHA was assessed by the methylthiazoltetrazolium (MTT) assay. Furthermore, microarray technology was applied to obtain the gene expression profiles of MSCs cultured on NHA at 24, 48, and 72 h. The gene expression profile was then comprehensively analyzed by clustering, Gene Ontology (GO), Gene Microarray Pathway Profiler (GenMAPP) and Ingenuity Pathway Analysis (IPA). According to the results of microarray experiment, 8992 differentially expressed genes were obtained. 90 differential expressed genes related to HA osteogenic differentiation were determined by GO analysis. These genes included not only 6 genes related to HA osteogenic differentiation as mentioned in the literatures but also newly discovered 84 genes. Some important signaling pathways (TGF‐β, MAPK, Wnt, etc.) were influenced by these genes. Gene interaction networks were obtained by IPA software, in which the scoring values of two networks were highest, and their main functions were related to cell development. The comprehensive analysis of these results indicate that NHA regulate some crucial genes (e.g., Bmp2, Spp1) and then activate some pathways such as TGF‐β signaling pathway, and ultimately osteogenic differentiation was induced. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2833–2841, 2014.